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At starting of experiments parasitaemia was set to approximately 2% PE. Blood smears were performed to confirm parasitaemia that varied from 1.9 to 2.1 (percentage of ring stages/ early trophozoite varied 87 to 97%). After 48h of culture all controls showed an increase in parasite number with total parasitaemia varying from 2.9 to 4.1. There were no statistical significant differences between control and treated parasites. However, we observed a decrease in the mean number of parasites when treated with 1μg mAb/ml. Anti-CSP-mAb had contradictory effect depending on concentration and although mAb at lower concentration seems to have a stimulatory effect, in this group the variability between experiments was high. The opposite was observed in parasites treated with IgG .. TET and its derivatives are among the most effective drugs against brucellosis [2]. DOX has become the most commonly prescribed tetracycline derivative in the treatment of brucella infections because of its superior pharmacokinetic features [28]. In the present study, among the tested antibacterial agents, DOX was found to have the lowest MIC50 and MIC90 values which is consistent with previous reports [8,10,11,22-24,27,29]. Conversely in a Mexican study, Lopez-Merino et al. found the MIC values for TET were higher than in Brucella strains isolated in Turkey [9] which demonstrates the antibiotic susceptibility patterns of Brucella strains appear to vary geographically.

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TET and its derivatives are among the most effective drugs against brucellosis [2]. DOX has become the most commonly prescribed tetracycline derivative in the treatment of brucella infections because of its superior pharmacokinetic features [28]. In the present study, among the tested antibacterial agents, DOX was found to have the lowest MIC50 and MIC90 values which is consistent with previous reports [8,10,11,22-24,27,29]. Conversely in a Mexican study, Lopez-Merino et al. found the MIC values for TET were higher than in Brucella strains isolated in Turkey [9] which demonstrates the antibiotic susceptibility patterns of Brucella strains appear to vary geographically..